Neuroscience Lecture by Robert Darnell (Howard Hughes Medical Institute, Rockefeller University New York, USA)

Title: "RNA Maps of the Brain"

Robert Darnell

Time and venue: 11.00 a.m. at the Lecture Hall (room 0.10 of the Max Planck Institute of Biophysics, Max-von-Laue-Str. 3, 60438 Frankfurt am Main, Campus Riedberg)

The brain has unique systems for regulating RNA metabolism, as exemplified by the neuron-specific RNA binding proteins Nova, Elavl, and RbFox3 (NeuN).  We developed the HITS-CLIP method to precisely map relevant protein-RNA interaction sites in living brain with single nucleotide resolution.  Recently, we have expanded HITS-CLIP to the study of the Fragile-X protein FMRP and Argonaute-microRNA-mRNA ternary interactions, enabling identification of translational control and microRNA binding sites on a transcriptome-wide scale in the brain, respectively.  Overlaying these different sets of maps reveals insights into the dynamics of RNA regulation in the brain, as well as aspects of protein-RNA interaction that do not conform to current understanding of their function.  These exceptions, and new experiments to address them at the single cell level, will be discussed.
Licatalosi et al. Nature 456, 464-9, 2008; Chi et al., Nature, 460:479-86, 2009; Zhang et al., Science 329:439-443, 2010, Darnell et al., Cell 146:247-61, 2011.  Iossifov et al., Neuron, in press.



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